Rat aortic smooth muscle cells labeled for F-actin (red) and phosphorylated caveolin (green) in focal adhesions. Courtesy of Dr. Kathy Griendling.

Lula L. Hilenski, Director

EMORY UNIVERSITY SCHOOL OF MEDICINE
Department of Medicine
Room 303, Woodruff Memorial Research Building
1639 Pierce Drive, Atlanta, GA 30322
Telephone (404)727-8116
FAX (404)727-3330
e-mail: lhilens@emory.edu

Overview

The Internal Medicine Imaging Core was established in 2000 as a core facility providing access to state-of-the-art imaging instrumentation and technologies on a shared-use basis to faculty, staff and students of the Division of Cardiology and Department of Medicine in the Emory University School of Medicine. The facility is located in the Woodruff Memorial Research Building (WMRB), and houses the following microscopes:

1. a Zeiss Axioskop microscope equipped with fluorescence optics and an AxioCam camera in Room 3312A
2. a Zeiss Axioskop microscope equipped with fluorescence optics and an RT Slider Spot camera in Room 3312A
3. an Olympus IX71 microscope equipped with fluorescence optics and an Olympus DP71 camera in Room 3312A
4. a Zeiss LSM 510 META confocal laser scanning microscope (CLSM) in Room 303.

In addition, WMRB 301 houses a fully equipped laboratory for processing of histological samples, including paraffin/frozen tissue embedding, sectioning, staining and immunohistochemistry using both chromogenic and fluorescence markers. The equipment includes:

1. Leica CM3050 cryostat
2. Microm HN 505E cryostat
3. Leica RM2035 and RM2235 microtomes
4. Leica EG1160 Tissue Embedding Station
5. Leica TP 1020 Automatic Tissue Processor with vacuum
6. Basic supplies and reagents for histology in Labconco Protector Work Station
7. Nikon Optiphot microscope
8. Fume hood

Additionally, in the vicinity of WMRB 301 on the third floor is equipment space housing four ultra low freezers for tissue/section storage and liquid N2 storage tanks for freezing specimens.

All potential users of the systems are asked to contact the director of the facility, Dr. Lula Hilenski, at the above address to schedule a one-on-one Basic Training session at a mutually convenient time. These individual training sessions, which usually last about 2 hours, include proper use and care of microscope objectives, operation of the microscope and laser system, laser safety, as well as how to acquire, save, export and transfer images.

Table of Contents:

• Overview
• Personnel
• Instrumentation
• Confocal Laser Scanning Microscopy
• Brightfield and Conventional Fluorescence Microscopy
• Guidelines for Use of the Facility
• Microscopes in the Imaging Core
• Instructions for Microscopes in the Imaging Core
• Zeiss LSM 510 META CLSM
• Zeiss Axioskop 2 Microscope
• Olympus IX71 Microscope
• Instructions for Histology Equipment
• Protocols
• Immunocytochemistry
• Immunohistochemistry
• Reactive Oxygen Species Detection
• How To…
• Image Analysis
• Lectures on Microscopy
• Ethics in Imaging
• Selected Publications from the Imaging Core
• Helpful Websites